Membranes were then incubated with the following primary antibodies diluted in the blocking solution: anti-AKT (#9272, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-AKT (Thr308) (#2965, 1:1000, Cell Signaling), anti-BDNF (sc-546, 1:500, Santa Cruz Biotechnology), anti-ERK1/2 (#9102, 1:1000, Cell Signaling), anti-phospho-ERK1/2 (Thr202/Tyr204) (#4370, 1:1000, Cell Signaling), anti-TrkB (#4603, 1:1000, Cell Signaling), anti-pTrkB (Tyr516) (#4619, 1:1000, Cell Signaling), anti--actin (#4970, 1:1000, Cell Signaling), anti-STAT3 (#9139, 1:1000, Cell Signaling), anti-p-STAT3 (#9145, 1:1000, Cell Signaling)
Age-matched non-Tg mice without any treatment were used as control
maintenance = 2.25 mg weekly - closely matching the standard protocol
Normal Physiology of Growth Hormone in Adults
You have read dozens of articles about GHK-Cu dosing but nobody actually showed you what to do when you are standing in your bathroom holding a needle for the first time
Presented for research literature review only Chang WC et al